Staining Reagents
Sigma Aldrich Fine Chemicals Biosciences BioTracker 488 Green Nucle
The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cells genetic material organized as multiple long linear DNA molecules in complex with a large variety of proteins such as histones to form chromosomes. Traditionally DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.The BioTracker 488 Green Nuclear Dye is a cell-membrane permeable green fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.Note BioTracker Nuclear Dyes also shows blue fluorescence in the DAPI channel and may not be suitable for multicolor imaging with blue probes.Spectral PropertiesAbsorbance 500nmEmis
Cytoskeleton, Inc. SiR-Tubulin Kit, 50 nmol SiR-Tubulin and 1 umol verapamil
SiR-tubulin is based on the microtubule binding drug Docetaxel. SiR-tubulin is fluorogenic, cell permeable and highly specific for microtubules. Sir-tubulin stains endogenous microtubules without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-tubulin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-tubulin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.
Biotium RedDot™2, 200X in DMSO
RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. The dye has an excitation/emission of 665/695 nm when bound to DNA. Properties: Liquid; Store at 4°C, protect from light; Ex/Em= 665/695 nm (with DNA).
Leica Biosystems 10% Neutral Buffered Formalin, 2.5 Gallon
10% Neutral Buffered Formalin is a ready-to-use fixative formulated for routine tissue fixation in histology and pathology laboratories. This product is supplied in a 2.5 gallon cube container for bulk laboratory use.
- Ready-to-use 10% neutral buffered formalin for tissue fixation.
- 2.5 gallon cube container (≈9.46 L) for bulk use.
- Suitable for routine histology and pathology applications.
- Premeasured volume reduces preparation time and variability.
- Packaged for storage and transport in laboratory environments.
Sigma Aldrich Fine Chemicals Biosciences BioTracker TiY Vimentin Li
Vimentin is an intermediate filament protein which is upregulated in cells undergoing epithelial-to-mesenchymal transition (EMT) and hence is commonly used an EMT marker. Tumor initiating cells (TICs) have been implicated in clinical relapse and metastasis of a variety of epithelial cancers including lung cancer.BioTracker TiY Vimentin is a live cell fluorescent dye that selectively stains TICs over differentiated tumor cells or normal cells. TiY can be used for visualization and enrichment of functionally active TICs from patient tumors. TiY displays better selectivity for tumorigenic cells than a CD166 antibody and can be used for TiY-staining-based cell sorting for TICs in various types of cancer (lung CNS melanoma breast renal ovarian colon and prostate cancer). At high concentrations TiY shows selective anti-TIC activity over non-TIC and normal tissue cells.Spectral PropertiesAbsorbance 553 nmEmission 573 nmReferencesLee Y et al. Identification of Tumor Initiating Cells w
Cayman Chemical Ruthenium Red 100mg
A polycationic dye that inhibits sarcoplasmic reticulum Ca2+ release, blocks Ca2+ uptake and release from mitochondria, and prevents Ca2+ release from ryanodine-sensitive intracellular stores in skeletal and cardiac muscle (Kis = 16 nm - 5.9 μM, depending on Ca2+ concentration)
eMolecules New Coccine | 25G | Ships from Wood Dale, IL | Chem-Impex | 2611-82-7 | MFCD00004084 | MW: 604.46
Chem-Impex | New Coccine | 25G | 2611-82-7 | MFCD19686984 | MW:563.654
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Bioventures Inc TRACKING DYE 6X 10ML
MAPMARKER, 50-1000BP, ROX Labeled (50, 75, 100, 125, 150, 200, 250, 300, 350, 400, 450, 475, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000bp), 8fmoles/band/ul Concentration
Sigma Aldrich Fine Chemicals Biosciences Reactive Blue 4 Dye content 35 % | 13324-20-4 | MFCD00001218 | 25G
Reactive Blue 4 Dye content 35 % | Mol Wt: 637.43 | 13324-20-4 | MFCD00001218 | 25G
Perkin Elmer US LLC GladiATR Heated Diamond Crystal Plate - 210° C
The GladiATR Vision optical design is all reflective, preserving the full spectral range inherent to diamond. For standard mid-IR, the spectral range available with the GladiATR Vision will be 400-400 cm-1. For Far-IR configuration, the spectral range is extended to less than 50 cm-1. The GladiATR Diamond ATR accessory is compatible with the Spectrum 3/Frontier spectrometers. It includes: Base Optics Monolithic diamond crystal plate High pressure clamp with three interchangeable tips Liquids retainer with volatiles cover Purge tubes Purge kit Spectrometer base mount
Sigma Aldrich Fine Chemicals Biosciences Disperse Blue 1 Dye content 30 % | 2475-45-8 | MFCD00001225 | 5G
Disperse Blue 1 Dye content 30 % | Mol Wt: 268.27 | 2475-45-8 | MFCD00001225 | 5G
Supply Solutions Trypan Blue solution 0.4% liquid sterile-filtered suitable for cell culture, 20mL
Trypan Blue solution 0.4% liquid sterile-filtered suitable for cell culture
American Research Products Inc Sheep C reactiVe protein CRP
Sheep C-reactiVe protein (CRP) ELISA Kit from CUSABIO Detection Range 0 625 g/mL-40 g/mL Sensitivity 0 156 g/mL Sample serum plasma tissue homogenates cell lysates Method Sandwich
American Research Products Inc Rat pigment epithelium deriVed
Rat pigment epithelium-deriVed factor PEDF ELISA Kit from CUSABIO Detection Range 6 25 pg/mL-400 pg/mL Sensitivity 1 56 pg/mL Sample serum plasma cell culture supernates tissue homogenates Method Sandwich